Analytical Data
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Gene name
ALDH4A1
- Application
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Alternative Names
ALDH4A1；ALDH4；P5CDH；Delta-1-pyrroline-5-carboxylate dehydrogenase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P30038
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Expression Region
25-563aa
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AA Sequence
KHTSSL KVANEPVLAF TQGSPERDAL QKALKDLKGR MEAIPCVVGD EEVWTSDVQY QVSPFNHGHK VAKFCYADKS LLNKAIEAAL AARKEWDLKP IADRAQIFLK AADMLSGPRR AEILAKTMVG QGKTVIQAEI DAAAELIDFF RFNAKYAVEL EGQQPISVPP STNSTVYRGL EGFVAAISPF NFTAIGGNLA GAPALMGNVV LWKPSDTAML ASYAVYRILR EAGLPPNIIQ FVPADGPLFG DTVTSSEHLC GINFTGSVPT FKHLWKQVAQ NLDRFHTFPR LAGECGGKNF HFVHRSADVE SVVSGTLRSA FEYGGQKCSA CSRLYVPHSL WPQIKGRLLE EHSRIKVGDP AEDFGTFFSA VIDAKSFARI KKWLEHARSS PSLTILAGGK CDDSVGYFVE PCIVESKDPQ EPIMKEEIFG PVLSVYVYPD DKYKETLQLV DSTTSYGLTG AVFSQDKDVV QEATKVLRNA AGNFYINDKS TGSIVGQQPF GGARASGTND KPGGPHYILR WTSPQVIKET HKPLGDWSYA YMQ
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ALDH4A1, or aldehyde dehydrogenase 4A1, is a crucial enzyme involved in the catabolism of various aldehydes and plays a significant role in the metabolism of amino acids, particularly proline and arginine. Mutations in the ALDH4A1 gene are linked to a rare genetic disorder known as pyrroline-5-carboxylate dehydrogenase deficiency, which can lead to neurological symptoms and developmental issues. Understanding the structure and function of ALDH4A1 is essential for elucidating its biological pathways and potential therapeutic targets. Recombinant ALDH4A1 protein has emerged as a valuable tool for biochemical studies, enabling researchers to investigate enzymatic activities, substrate specificity, and the effects of various mutations. Such studies not only enhance our comprehension of its role in cellular metabolism but also pave the way for developing novel treatments for related metabolic disorders. The production and characterization of recombinant ALDH4A1 can provide insights into its functional mechanism and interactions with other metabolic pathways, ultimately contributing to improved diagnostic and therapeutic strategies for patients affected by ALDH4A1-related conditions. Enhanced research efforts in this area can lead to the identification of biomarkers for disease progression and therapeutic interventions that target the underlying enzymatic deficiencies.
