Analytical Data
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Gene name
CCNE1
- Application
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Alternative Names
CCNE1；CCNE；G1/S-specific cyclin-E1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P24864
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Expression Region
1-410aa
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AA Sequence
MPRERRERDA KERDTMKEDG GAEFSARSRK RKANVTVFLQ DPDEEMAKID RTARDQCGSQ PWDNNAVCAD PCSLIPTPDK EDDDRVYPNS TCKPRIIAPS RGSPLPVLSW ANREEVWKIM LNKEKTYLRD QHFLEQHPLL QPKMRAILLD WLMEVCEVYK LHRETFYLAQ DFFDRYMATQ ENVVKTLLQL IGISSLFIAA KLEEIYPPKL HQFAYVTDGA CSGDEILTME LMIMKALKWR LSPLTIVSWL NVYMQVAYLN DLHEVLLPQY PQQIFIQIAE LLDLCVLDVD CLEFPYGILA ASALYHFSSS ELMQKVSGYQ WCDIENCVKW MVPFAMVIRE TGSSKLKHFR GVADEDAHNI QTHRDSLDLL DKARAKKAML SEQNRASPLP SGLLTPPQSG KKQSSGPEMA
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CCNE1, or Cyclin E1, is a critical regulator of the cell cycle, particularly during the transition from the G1 phase to the S phase, where it coordinates the initiation of DNA replication. Abnormal expression of CCNE1 has been implicated in various cancers, highlighting its role in tumorigenesis. Research shows that CCNE1 can promote cell proliferation and contribute to genomic instability when overexpressed, making it a potential biomarker for cancer diagnosis and prognosis. Given its significance in cancer biology, the production and study of recombinant CCNE1 proteins have become essential for understanding its mechanistic role in cell cycle regulation and its interactions with cyclin-dependent kinases (CDKs). Recombinant techniques allow for the generation of large quantities of pure CCNE1 protein, facilitating the exploration of its structural and functional properties. These studies can reveal insights into how CCNE1 influences cellular processes and its potential as a therapeutic target. Overall, the investigation of CCNE1 as a recombinant protein serves not only to elucidate its biological functions but also to advance cancer research and therapeutic development strategies.
