Analytical Data
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Gene name
SPT16
- Application
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Alternative Names
SPT16；FACT140；FACTP140；FACT complex subunit SPT16
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O82491
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Expression Region
890-1074aa
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AA Sequence
DFKKDVLRVDSVPTSSLEGIKEWLDTTDIKYYESKLNLNWRQILKTITDDPQSFIDDGGWEFLNLDGSDSESGGSEESDKGYEPSDVEVESESEDEASESESLVESDDDEEEDSEQESEEEKGKTWDELEREATNADREHGVESDSEEERKRRKMKAFGKSRPGTSGGGGSSSMKNMPPSKRKHR
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Molecular Weight
22.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPT16, a crucial component of the FACT (Facilitates Chromatin Transcription) complex, plays a significant role in the regulation of transcription by modulating chromatin structure during RNA polymerase II transcription. Recent studies have highlighted the importance of SPT16 in maintaining genome stability and facilitating efficient transcriptional elongation. Understanding its function is pivotal for insights into cellular processes such as gene expression, DNA repair, and chromatin organization. The investigation of SPT16 recombinant proteins has gained traction, as these proteins can be utilized in biochemical assays to elucidate their interactions with histones and other chromatin-associated factors. Additionally, the study of SPT16 provides a paradigm for exploring the molecular mechanisms that underpin transcriptional regulation and chromatin dynamics, which are crucial for normal cell function and differentiation. Dysregulation of these processes can lead to various diseases, including cancer. Therefore, recombinant SPT16 proteins are not only valuable tools for dissecting its biological roles but also have the potential to uncover therapeutic targets for treating disorders associated with transcriptional dysregulation. As research advances, further elucidation of SPT16’s structure and function may provide new insights into the complex interplay between transcription and chromatin remodeling, contributing to our overall understanding of genomic integrity and expression regulation.
