Analytical Data
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基因名
RAD23A
- Application
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别名
RAD23A；UV excision repair Protein RAD23 homolog A
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54725
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表达区间
1-362aa
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氨基酸序列
MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPATSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE
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分子量
47.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAD23A is a pivotal protein involved in various cellular processes, particularly in the response to DNA damage and the regulation of proteostasis. As a member of the RAD23 family, RAD23A plays a crucial role in the nucleotide excision repair (NER) pathway, which is essential for repairing bulky DNA lesions that can arise from environmental factors such as UV radiation and chemical agents. Studies have shown that RAD23A interacts with several key proteins in the NER machinery, facilitating the recognition and repair of damaged DNA. Additionally, RAD23A is implicated in the ubiquitin-proteasome system, where it assists in the degradation of misfolded or damaged proteins, thus maintaining cellular health and function. Dysregulation of RAD23A has been linked to various diseases, including cancer, where impaired DNA repair mechanisms can lead to genomic instability. Given its significant role in maintaining genome integrity and cellular homeostasis, understanding the structure, function, and regulation of RAD23A is vital for developing therapeutic strategies aimed at enhancing DNA repair processes and targeting malignancies associated with its dysfunction. Recent advances in recombinant protein technology have enabled the production of purified RAD23A for detailed biochemical and structural studies, paving the way for deeper insights into its mechanisms of action and potential as a therapeutic target.
