Analytical Data
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基因名
VCL
- Application
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别名
VCL;Vinculin
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18206
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表达区间
2-235aa
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氨基酸序列
PVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVE
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分子量
53.0kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Vibrio cholerae lectin (VCL) is a significant research subject due to its role in the pathogenicity of Vibrio cholerae, the bacterium responsible for cholera. VCL facilitates the adhesion of the bacterium to intestinal epithelial cells, which is a crucial step in the infection process. The study of VCL and its recombinant proteins has garnered attention not only for understanding the mechanisms of cholera pathogenesis but also for developing potential therapeutic interventions and vaccines. Utilizing recombinant DNA technology, researchers can produce VCL in a controlled manner, enabling detailed functional analyses and the exploration of its interactions with host cells. This approach allows for the investigation of VCL's structure-function relationships, providing insights into its binding affinities and the molecular underpinnings of its activity. Additionally, the recombinant VCL has potential applications in biomedicine, including as a diagnostic tool for cholera or as a platform for drug delivery systems. By elucidating the properties and behavior of VCL in vitro and in vivo, researchers aim to pave the way for new strategies in combating cholera and improving public health outcomes in regions disproportionately affected by this disease. Overall, the study of VCL recombinant proteins stands at the intersection of microbiology, immunology, and biotechnology, presenting opportunities to advance our understanding of cholera and enhance global health initiatives.












