Cat: PA1000-4783

Recombinant Human SMPDL3A Protein,His

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Analytical Data

  • Gene name

    SMPDL3A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    SMPDL3A;ASML3A;Cyclic GMP-AMP phosphodiesterase SMPDL3A

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q92484

  • Expression Region

    23-453aa

  • AA Sequence

    LPVAPAGGRNPPPAIGQFWHVTDLHLDPTYHITDDHTKVCASSKGANASN PGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELST DTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVA NLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLN KTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYN EKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPV KSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEY ILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDK TCKAFQICAIMNLDNISYADCLKQLYIKHNYVDHHHHHH

  • Molecular Weight

    50 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

SMPDL3A, or sphingomyelin phosphodiesterase acid-like 3A, is an intriguing member of the SMPD gene family, which plays a pivotal role in lipid metabolism and cellular signaling. Research has indicated that SMPDL3A is involved in sphingolipid metabolism, influencing various biological processes including cell proliferation, apoptosis, and inflammation. Abnormal expression or dysfunction of SMPDL3A has been linked to several pathological conditions, such as cancer and neurodegenerative diseases. Due to its substantial impact on cellular homeostasis, understanding the structure and function of recombinant SMPDL3A proteins has become a focal point in biomedical research. The generation of recombinant SMPDL3A offers valuable insights into its enzymatic activity, substrate specificity, and regulatory mechanisms. Furthermore, studying the protein's interactions with other cellular components may reveal novel therapeutic targets for diseases associated with sphingolipid dysregulation. By analyzing recombinant SMPDL3A, researchers aim to elucidate its role in health and disease, potentially leading to innovative strategies for treatment and prevention.

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