Analytical Data
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Gene name
HXK1
- Application
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Alternative Names
HXK1；Hexokinase-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19367
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Expression Region
13-475aa
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AA Sequence
ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA
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Molecular Weight
55.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HXK1 (Hexokinase 1) is an essential enzyme that plays a pivotal role in glucose metabolism by catalyzing the phosphorylation of glucose to glucose-6-phosphate. This step is a key regulatory point in glycolysis and serves as a link between carbohydrate metabolism and various cellular processes, including energy production and signaling. Research on recombinant HXK1 has gained traction due to its potential implications in understanding metabolic disorders, cancer progression, and diabetes. Moreover, abnormal HXK1 activity has been associated with various diseases, making it an attractive target for therapeutic interventions. Advances in recombinant DNA technology have enabled the production of HXK1 in heterologous systems, allowing for detailed studies of its enzymatic properties, structural characteristics, and interactions with other metabolic pathways. Through the expression of HXK1 as a recombinant protein, researchers can investigate the enzyme's kinetic properties, substrate specificity, and regulatory mechanisms. Furthermore, understanding the modulation of HXK1 activity can provide insights into its role in cellular homeostasis and its potential as a biomarker or drug target in metabolic diseases. Consequently, the study of recombinant HXK1 offers valuable avenues for both basic research and the development of novel pharmacological strategies.
