Analytical Data
-
Gene name
THOP1
- Application
-
Alternative Names
THOP1；Thimet oligopeptidase
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P52888
-
Expression Region
2-689aa
-
AA Sequence
KPPAACAGDMADAASPCSVVNDLRWDLSAQQIEERTRELIEQTKRVYDQV GTQEFEDVSYESTLKALADVEVTYTVQRNILDFPQHVSPSKDIRTASTEA DKKLSEFDVEMSMREDVYQRIVWLQEKVQKDSLRPEAARYLERLIKLGRR NGLHLPRETQENIKRIKKKLSLLCIDFNKNLNEDTTFLPFTLQELGGLPE DFLNSLEKMEDGKLKVTLKYPHYFPLLKKCHVPETRRKVEEAFNCRCKEE NCAILKELVTLRAQKSRLLGFHTHADYVLEMNMAKTSQTVATFLDELAQK LKPLGEQERAVILELKRAECERRGLPFDGRIRAWDMRYYMNQVEETRYCV DQNLLKEYFPVQVVTHGLLGIYQELLGLAFHHEEGASAWHEDVRLYTARD AASGEVVGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQIAIAAMVAN FTKPTADAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFV EAPSQMLENWVWEQEPLLRMSRHYRTGSAVPRELLEKLIESRQANTGLFN LRQIVLAKVDQALHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGHL AGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLNSKVGMDYRSCILRPGGS EDASAMLRRFLGRDPKQDAFLLSKGLQVGGCEPEPQVC
-
Molecular Weight
79 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
THOP1 (Thimet Oligopeptidase 1) is a metallopeptidase that plays a crucial role in the degradation of bioactive peptides, influencing various physiological processes, including neuropeptide metabolism and immune responses. Characterized by its ability to cleave peptide bonds at the N-terminus of short peptides, THOP1 is involved in the regulation of neuropeptides that affect pain, mood, and stress responses, making it a target of interest in studying neurodegenerative diseases and psychological disorders. Research has highlighted its potential implications in cancer biology, as altered THOP1 expression levels can affect tumor growth and metastasis through modulation of peptide signaling pathways. Despite its significance, the structural and functional characterizations of THOP1 remain limited. Recent advances in recombinant protein technology provide opportunities to produce and purify THOP1 for in-depth studies. The generation of recombinant THOP1 enables researchers to investigate its enzymatic activity, substrate specificity, and regulatory mechanisms, which could uncover potential therapeutic targets for diseases linked to THOP1 dysregulation. As understanding the biochemical properties of THOP1 can aid in elucidating its precise role in cellular processes, ongoing studies are anticipated to enhance our comprehension of its function in health and disease.
