Analytical Data
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Gene name
CHIT1
- Application
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Alternative Names
CHIT1；Chitotriosidase-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13231
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Expression Region
22-466aa
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AA Sequence
AKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTEW NDETLYQEFNGLKKMNPKLKTLLAIGGWNFSTQKFTDMVATANNRQTFVN SAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQ TSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKV TGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSF TLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQK VPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCN QGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQ GKADGLYPNPRERSSFYSCAGGRLFQQSCPTGLVFSNSCKCCTWNVDHHH HHH
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Molecular Weight
50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ChIT1, short for Chitinase 1, is an important glycoprotein that plays a crucial role in chitin metabolism and has been linked to various physiological and pathological processes. Research on ChIT1 has gained attention due to its involvement in tissue remodeling, inflammation, and its potential as a biomarker for diseases such as asthma, cancer, and neurodegenerative disorders. The study of recombinant ChIT1 protein has become increasingly relevant, as it allows for the elucidation of its functional properties, enzymatic activity, and interaction with other biomolecules. Understanding the structure-function relationship of ChIT1 can provide insights into its biological significance and therapeutic potentials. Furthermore, as recombinant techniques advance, producing ChIT1 in sufficient quantities for experimental purposes has become feasible, paving the way for in-depth investigations into its role in various biological systems and its implications in disease mechanisms. The insights gained from these studies may lead to novel therapeutic strategies and enhance our understanding of chitinase biology in health and disease.
