Analytical Data
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Gene name
C14orf149
- Application
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Alternative Names
L3HYPDH; C14orf149; Trans-3-hydroxy-L-proline dehydratase; EC 4.2.1.77; Trans-L-3-hydroxyproline dehydratase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96EM0
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Expression Region
1-354 aa
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AA Sequence
MESALAVPRLPPHDPGTPVLSVVDMHTGGEPLRIVLAGCPEVSGPTLLAKRRYMRQHLDHVRRRLMFEPRGHRDMYGAVLVPSELPDAHLGVLFLHNEGYSSMCGHAVLALGRFALDFGLVPAPPAGTREARVNIHCPCGLVTAFVACEDGRSHGPVRFHSVPAFVLATDLMVDVPGHGKVMVDIAYGGAFYAFVTAEKLGLDICSAKTRDLVDAASAVTEAVKAQFKINHPDSEDLAFLYGTILTDGKDAYTKEPTTNICVFADEQVDRSPTGSGVTARIALQYHKGLLELNQMRAFKSSATGSVFTGKAVREAKCGDFKAVIVEVSGQAHYTGTASFIIEDDDPLRDGFLLK
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Molecular Weight
64.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
C14orf149, a gene located on chromosome 14, has garnered attention due to its potential role in various biological processes and diseases. Research indicates that C14orf149 may be involved in cellular functions such as proliferation, differentiation, and apoptosis. Studies suggest it might be implicated in several pathological conditions, including cancer, where its expression levels can correlate with tumor progression and patient prognosis. The lack of extensive functional characterization has led scientists to explore recombinant protein production of C14orf149. This approach allows researchers to investigate the protein's structure, function, and interactions in a controlled environment. By utilizing techniques such as molecular cloning and protein expression systems, scientists aim to produce sufficient quantities of the protein to facilitate biochemical assays and structural studies. Understanding the role of C14orf149 through recombinant protein research could provide insights into its mechanisms in cellular processes and its potential as a therapeutic target or biomarker in diseases.
