Analytical Data
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Gene name
PRKCB
- Application
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Alternative Names
KPCB_HUMAN; PKC Beta; PKC-B; PKC-beta; PKCB; Prkcb; PRKCB II; PRKCB2; Protein kinase C beta; Protein kinase C beta type
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P05771
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Expression Region
2-671 aa
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AA Sequence
ADPAAGPPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS KHKFKIHTYS SPTFCDHCGS LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL CGTDHTERRG RIYIQAHIDR DVLIVLVRDA KNLVPMDPNG LSDPYVKLKL IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL SVEIWDWDLT SRNDFMGSLS FGISELQKAS VDGWFKLLSQ EEGEYFNVPV PPEGSEANEE LRQKFERAKI SQGTKVPEEK TTNTVSKFDN NGNRDRMKLT DFNFLMVLGK GSFGKVMLSE RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKEHAFF RYIDWEKLER KEIQPPYKPK ARDKRDTSNF DKEFTRQPVE LTPTDKLFIM NLDQNEFAGF SYTNPEFVIN V
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Molecular Weight
76.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRKCB, or protein kinase C beta, is a member of the protein kinase C (PKC) family, which plays a crucial role in various cellular processes, including proliferation, differentiation, and apoptosis. Dysregulation of PRKCB has been associated with several diseases, including cancer, cardiovascular disorders, and neurological conditions. The study of PRKCB recombinant proteins is significant for several reasons. First, understanding its structure and function can shed light on the molecular mechanisms of diseases where PRKCB is implicated. Second, the use of recombinant PRKCB can facilitate the development of targeted therapies, allowing for the design of inhibitors that can modulate its activity and potentially reverse pathological states. Furthermore, PRKCB's role in signaling pathways makes it an attractive target for drug development, as small molecules or biologics that can selectively influence its activity might lead to novel treatment strategies. Researchers are utilizing advanced techniques, such as X-ray crystallography and NMR spectroscopy, to elucidate the structure-function relationships of PRKCB. Additionally, the expression and purification of recombinant PRKCB enable comprehensive biochemical and pharmacological studies, providing insights into how it interacts with other cellular components. Overall, the research surrounding PRKCB recombinant proteins is vital for advancing our understanding of cellular signaling and developing new therapeutic interventions for diseases linked to PRKCB dysregulation.
