Analytical Data
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基因名
ATP5F1B
- Application
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别名
ATP5F1B；ATP5B；ATPMB；ATPSB；ATP synthase subunit beta. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P06576
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表达区间
48-529aa
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氨基酸序列
AQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS
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分子量
58.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP5F1B, a subunit of ATP synthase, plays a crucial role in mitochondrial function by facilitating ATP production through oxidative phosphorylation. Research on ATP5F1B recombinant protein is significant due to its potential implications in understanding energy metabolism and its association with various diseases, including neurodegenerative disorders and metabolic syndromes. The importance of ATP5F1B lies in its contribution to the mitochondrial complex V, where it aids in the assembly and stability of the ATP synthase enzyme complex. Abnormalities in ATP5F1B have been linked to impaired mitochondrial function, leading to a spectrum of pathologies stemming from bioenergetic deficits. Thus, the recombinant expression of ATP5F1B allows for in-depth studies regarding its biochemical properties, interactions with other mitochondrial proteins, and overall impact on cellular energy dynamics. Investigating ATP5F1B through recombinant technology also opens avenues for therapeutic strategies aimed at restoring normal mitochondrial function and mitigating disease progression related to mitochondrial dysfunction. By elucidating the structure-function relationship of this protein, researchers aim to develop targeted interventions that can enhance ATP synthase activity and improve cellular bioenergetics in affected tissues. Overall, the study of ATP5F1B recombinant protein serves as a vital component in the broader effort to understand mitochondrial biology and its clinical relevance in human health.
