Analytical Data
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Gene name
ATP5F1A
- Application
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Alternative Names
ATP5F1A；ATP5A；ATP5A1；ATP5AL2；ATP synthase subunit alpha. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P25705
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Expression Region
44-553aa
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AA Sequence
QKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA
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Molecular Weight
59.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP5F1A, also known as ATP synthase subunit alpha, is a crucial component of the mitochondrial ATP synthase complex, which plays a vital role in ATP production through oxidative phosphorylation. This enzyme complex is essential for cellular energy metabolism, as it catalyzes the synthesis of ATP from ADP and inorganic phosphate using the proton gradient generated by the electron transport chain. Dysfunctional ATP5F1A has been implicated in various mitochondrial diseases, neurodegenerative disorders, and metabolic syndromes, highlighting its significance in maintaining cellular energy homeostasis. Recent studies have focused on the recombinant expression of ATP5F1A to facilitate structural and functional analyses, enabling researchers to understand the mechanisms underlying ATP synthase activity and its regulation. The development of recombinant ATP5F1A proteins allows for the investigation of potential therapeutic targets and the exploration of strategies to alleviate mitochondrial dysfunction. Overall, the study of ATP5F1A not only contributes to our understanding of mitochondrial biology but also opens avenues for the development of novel interventions in diseases associated with energy metabolism.
