Analytical Data
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基因名
PPM1L
- Application
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别名
PPM1L; PP2CE; Protein phosphatase 1L; EC 3.1.3.16; Protein phosphatase 1-like; Protein phosphatase 2C isoform epsilon; PP2C-epsilon
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5SGD2
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表达区间
1-360 aa
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氨基酸序列
MIEDTMTLLSLLGRIMRYFLLRPETLFLLCISLALWSYFFHTDEVKTIVKSSRDAVKMVKGKVAEIMQNDRLGGLDVLEAEFSKTWEFKNHNVAVYSIQGRRDHMEDRFEVLTDLANKTHPSIFGIFDGHGGETAAEYVKSRLPEALKQHLQDYEKDKENSVLSYQTILEQQILSIDREMLEKLTVSYDEAGTTCLIALLSDKDLTVANVGDSRGVLCDKDGNAIPLSHDHKPYQLKERKRIKRAGGFISFNGSWRVQGILAMSRSLGDYPLKNLNVVIPDPDILTFDLDKLQPEFMILASDGLWDAFSNEEAVRFIKERLDEPHFGAKSIVLQSFYRGCPDNITVMVVKFRNSSKTEEQ
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分子量
67.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPM1L (Protein Phosphatase, Magnesium Dependent 1L) is a member of the protein phosphatase family, specifically functioning as a magnesium-dependent serine/threonine phosphatase. Its role is critical in various cellular processes, including cell signaling, stress response, and the regulation of the cell cycle. Research has shown that PPM1L plays a significant role in dephosphorylating target proteins involved in key signaling pathways such as MAPK and PI3K/Akt. Dysregulation of PPM1L has been linked to several diseases, including cancer and metabolic disorders, making it an important target for therapeutic interventions. Additionally, PPM1L is implicated in the modulation of immune responses and the regulation of apoptosis, highlighting its potential influence on both innate and adaptive immunity. Given its central role in various biological processes, the recombinant production of PPM1L is essential for studying its functional mechanisms, post-translational modifications, and interactions with other cellular proteins. The ability to produce PPM1L as a recombinant protein allows for the exploration of its structural characteristics and enzymatic activities, providing insights into its biological roles and potential as a drug target. Understanding PPM1L at the molecular level could lead to novel approaches for treating diseases associated with its dysfunction, representing a promising avenue in biomedical research.
