Analytical Data
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Gene name
POU4F3
- Application
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Alternative Names
Brain specific homeobox/POU domain protein 3C; Brain-3C; Brain-specific homeobox/POU domain protein 3C; BRN 3C; Brn-3C; BRN3C; class 4; DFNA 15; DFNA15; MGC138412; PO4F3_HUMAN; POU class 4 homeobox 3; POU domain; POU domain class 4 transcription factor 3; POU4F3; transcription factor 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15319
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Expression Region
1-338 aa
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AA Sequence
MMAMNSKQPF GMHPVLQEPK FSSLHSGSEA MRRVCLPAPQ LQGNIFGSFD ESLLARAEAL AAVDIVSHGK NHPFKPDATY HTMSSVPCTS TSSTVPISHP AALTSHPHHA VHQGLEGDLL EHISPTLSVS GLGAPEHSVM PAQIHPHHLG AMGHLHQAMG MSHPHTVAPH SAMPACLSDV ESDPRELEAF AERFKQRRIK LGVTQADVGA ALANLKIPGV GSLSQSTICR FESLTLSHNN MIALKPVLQA WLEEAEAAYR EKNSKPELFN GSERKRKRTS IAAPEKRSLE AYFAIQPRPS SEKIAAIAEK LDLKKNVVRV WFCNQRQKQK RMKYSAVH
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Molecular Weight
37.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POU4F3, also known as Brn-3c, is a member of the POU family of transcription factors, which play critical roles in the development and function of sensory neurons, particularly in the inner ear and retina. Research has identified POU4F3 as essential for the differentiation and survival of these neurons, making it a significant focus in studies related to hearing loss and vision disorders. Mutations in the POU4F3 gene have been linked to autosomal dominant hearing loss, underscoring its importance in auditory pathways. The understanding of POU4F3's molecular mechanisms has been advanced through the study of its protein structure, regulatory elements, and interaction with other transcription factors. In recent years, the recombinant expression of POU4F3 protein has facilitated the exploration of its functional properties and interactions in cellular models. This has paved the way for potential therapeutic approaches, aimed at mitigating the impact of genetic mutations on neuronal development. As researchers continue to investigate the role of POU4F3 in neural development, its implications in regenerative medicine and gene therapy offer promising avenues for future studies aimed at repairing auditory and visual functions compromised by genetic abnormalities. Overall, the ongoing exploration of POU4F3 as a reconstituted protein holds significant promise for understanding and treating various sensory deficits linked to its dysregulation.
