Analytical Data
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Gene name
POU1F1
- Application
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Alternative Names
Dwarf; GHF 1; GHF 1A; GHF-1; GHF1; GHF1A; Growth hormone factor 1; Hmp 1; Hmp1; Pit 1; Pit 1 beta; PIT 1Z; PiT-1; Pit1 beta; PIT1_HUMAN; PIT1Z; Pituitary growth hormone; Pituitary specific positive transcription factor 1; Pituitary-specific positive transcription factor 1; POU class 1 homeobox 1; POU domain class 1 transcription factor 1; POU domain transcriptional regulator; POU1F1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P28069
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Expression Region
1-291 aa
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AA Sequence
MSCQAFTSAD TFIPLNSDAS ATLPLIMHHS AAECLPVSNH ATNVMSTATG LHYSVPSCHY GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPIHQPLLAE DPTAADFKQE LRRKSKLVEE PIDMDSPEIR ELEKFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS SQEIMRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R
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Molecular Weight
32.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POU1F1, also known as Pit-1, is a transcription factor that plays a crucial role in the development and function of the pituitary gland. This protein is involved in stimulating the expression of growth hormone (GH), prolactin (PRL), and thyroid-stimulating hormone (TSH), making it essential for normal endocrine activity and growth regulation. Mutations in the POU1F1 gene can lead to combined pituitary hormone deficiency (CPHD), resulting in various developmental issues and endocrine disorders. Research on POU1F1 recombinant protein focuses on understanding its structural characteristics and functional mechanisms in gene regulation. The recombinant production of this protein enables detailed studies on its binding interactions, protein-protein interactions, and potential as a therapeutic target for treating pituitary disorders. Given its significant implications in growth and metabolism, POU1F1 continues to be a focal point of investigation in both basic and clinical research, with potential applications in gene therapy and regenerative medicine. Understanding the intricacies of POU1F1's role in endocrine signaling can provide insights into novel treatment strategies for hormone-related diseases.
