Analytical Data
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Gene name
USP7
- Application
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Alternative Names
USP7；HAUSP；Ubiquitin carboxyl-terminal hydrolase 7
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q93009
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Expression Region
2-1102aa
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AA Sequence
MHHHHHHDYKDDDDKNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRI TQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVL SPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQA VLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDD DKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTN QLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWE TLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKE VDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGL QEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEF LQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVS RCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDI PQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDE EKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPA MLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVM LFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYE EVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEY FRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPML LQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKIT DFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKAS GKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKEN EMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFE KFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAP KRSRYTYLEKAIKIHN
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Molecular Weight
130 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USP7 (Ubiquitin Specific Peptidase 7), also known as HAUSP (Herpesvirus-Associated Ubiquitin Specific Protease), is a deubiquitinating enzyme that plays a pivotal role in the regulation of various cellular processes, including protein degradation, signal transduction, and DNA damage response. It is known to interact with key tumor suppressors such as p53 and PTEN, thereby influencing cell cycle progression and apoptosis. The dysregulation of USP7 has been implicated in various cancers, making it a potential therapeutic target. Recent studies have emphasized the importance of USP7 in the modulation of cell signaling pathways and its involvement in viral infections, as certain viruses exploit USP7 to maintain their lifecycle by manipulating host cell pathways. Research into the recombinant production of USP7 protein has gained traction, as it allows for the study of its biochemical properties, substrate specificity, and interaction with small molecule inhibitors. The ability to produce USP7 in a recombinant system facilitates high-throughput screenings for drugs that can inhibit its activity, presenting a promising avenue for cancer therapy. Thus, understanding the functional mechanisms of USP7 through recombinant protein studies is critical for developing novel strategies to combat tumors that exploit this enzyme's regulatory functions.
