Analytical Data
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Gene name
BRDG1
- Application
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Alternative Names
BCR downstream signaling Protein 1; BCR downstream-signaling Protein 1; BRDG 1; BRDG1; Docking Protein BRDG1; Signal transducing adaptor family member 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9ULZ2
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Expression Region
1-295aa
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AA Sequence
MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLIKRSGYR EYEHYWTELR GTTLFFYTDK KSIIYVDKLD IVDLTCLTEQ NSTEKNCAKF TLVLPKEEVQ LKTENTESGE EWRGFILTVT ELSVPQNVSL LPGQVIKLHE VLEREKKRRI ETEQSTSVEK EKEPTEDYVD VLNPMPACFY TVSRKEATEM LQKNPSLGNM ILRPGSDSRN YSITIRQEID IPRIKHYKVM SVGQNYTIEL EKPVTLPNLF SVIDYFVKET RGNLRPFICS TDENTGQEPS MEGRSEKLKK NPHIA
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Molecular Weight
60.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BRDG1, also known as BRI1-Associated Receptor Kinase 1, is a crucial protein involved in various cellular processes, particularly in the context of plant immune response and development. Research has shown that BRDG1 plays a significant role in the signaling pathways activated by brassinosteroids, which are vital plant hormones regulating growth and stress responses. Understanding the function and mechanisms of BRDG1 is essential for elucidating the complex networks that govern plant biology. Recent studies have indicated that BRDG1 not only contributes to brassinosteroid signaling but also interacts with various other proteins, indicating a multifunctional role in cellular signaling. Investigating the structure, function, and interaction of BRDG1 through recombinant protein techniques can provide valuable insights into its biological significance and potential applications in agricultural biotechnologies. With ongoing challenges in global food security and crop resilience against biotic and abiotic stresses, elucidating the roles of proteins like BRDG1 can pave the way for developing improved plant varieties with enhanced growth and resistance traits. This research area continues to attract attention as scientists aim to harness the potential of BRDG1 for innovative agricultural solutions.











