Analytical Data
-
Gene name
PLB1
- Application
-
Alternative Names
PLB1; PLB; Phospholipase B1; membrane-associated; Phospholipase B; hPLB; Phospholipase B/lipase; PLB/LIP) [Includes: Phospholipase A2; EC 3.1.1.4); Lysophospholipase; EC 3.1.1.5)]
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q6P1J6
-
Expression Region
1-423 aa
-
AA Sequence
MPITCPTQNEPFLRTPRNSNYTYPIKPAIENWGSDFLCTEWKASNSVPTSVHQLRPADIKVVAALGDSLTTAVGARPNNSSDLPTSWRGLSWSIGGDGNLETHTTLPNILKKFNPYLLGFSTSTWEGTAGLNVAAEGARARDMPAQAWDLLERMKNSPDINLEKDWKLVTLFIGVNDLCHYCENPEAHLATEYVQHIQQALDILSEELPRAFVNVVEVMELASLYQGQGGKCAMLAAQNNCTCLRHSQSSLEKQELKKVNWNLQHGISSFSYWHQYTQREDFAVVVQPFFQNTLTPLNERGDTDLTFFSEDCFHFSDRGHAEMAIALWNNMLEPVGRKTTSNNFTHSRAKLKCPSPESPYLYTLRNSRLLPDQAEEAPEVLYWAVPVAAGVGLVVGIIGTVVWRCRRGGRREDPPMSLRTVAL
-
Molecular Weight
73.8 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PLB1, or Phospholipase B1, is an important enzyme implicated in various biological processes, including membrane lipid metabolism and cell signaling. Its dual enzyme activity allows it to hydrolyze both phospholipids and triglycerides, making it a crucial player in lipid homeostasis and inflammatory responses. Research into PLB1 has gained momentum due to its potential role in disease mechanisms, particularly in conditions such as obesity, diabetes, and cancer. Understanding PLB1's structure, function, and regulatory mechanisms can reveal insights into its involvement in these diseases, providing potential targets for therapeutic interventions. Additionally, recombinant PLB1 proteins offer valuable tools for biochemical studies, enabling researchers to investigate its enzymatic activity, substrate specificity, and interactions with other cellular components. The advancement of techniques in molecular biology and protein engineering has facilitated the production and characterization of PLB1, enhancing our ability to unravel its physiological significance and therapeutic potential.
