Analytical Data
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Gene name
BHLHB3
- Application
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Alternative Names
Basic helix-loop-helix domain containing class B3; BHE41_HUMAN; bHLH Protein DEC2; bHLHB3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9C0J9
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Expression Region
1-482aa
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AA Sequence
MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSMKRDD TKDTYKLPHR LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT ALTEQQHQKI IALQNGERSL KSPIQSDLDA FHSGFQTCAK EVLQYLSRFE SWTPREPRCV QLINHLHAVA TQFLPTPQLL TQQVPLSKGT GAPSAAGSAA APCLERAGQK LEPLAYCVPV IQRTQPSAEL AAENDTDTDS GYGGEAEARP DREKGKGAGA SRVTIKQEPP GEDSPAPKRM KLDSRGGGSG GGPGGGAAAA AAALLGPDPA AAAALLRPDA ALLSSLVAFG GGGGAPFPQP AAAAAPFCLP FCFLSPSAAA AYVQPFLDKS GLEKYLYPAA AAAPFPLLYP GIPAPAAAAA AAAAAAAAAA AFPCLSSVLS PPPEKAGAAA ATLLPHEVAP LGAPHPQHPH GRTHLPFAGP REPGNPESSA QEDPSQPGKE AP
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Molecular Weight
50.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BHLHB3 (Basic Helix-Loop-Helix Family Member B3) is a transcription factor that plays a critical role in various biological processes, including neurogenesis, cell differentiation, and apoptosis. Its unique structure, characterized by a basic helix-loop-helix motif, allows it to bind DNA and regulate gene expression. Research into BHLHB3 has gained significance due to its involvement in central nervous system development and function, as well as its potential link to neurodevelopmental disorders and cancers. Studies have demonstrated that BHLHB3 is essential for the proper formation and maintenance of neuronal circuits, implicating it in disorders like schizophrenia and autism spectrum disorders. The recombinant protein of BHLHB3 enables detailed investigation into its biochemical properties, signaling pathways, and interactions with other cellular components. Understanding the mechanisms governing BHLHB3 function could lead to novel therapeutic strategies for treating related neurological conditions. Recent advances in protein expression and purification techniques have facilitated the generation of functional BHLHB3 protein, providing an invaluable tool for researchers to explore its role in gene regulation and cellular processes. Furthermore, the study of BHLHB3 and its pathways could contribute to the broader understanding of transcriptional networks essential for normal brain development and function.
