Analytical Data
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Gene name
HARS1
- Application
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Alternative Names
HARS1；HARS；HRS；Histidine--tRNA ligase. cytoplasmic
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P12081
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Expression Region
2-504aa
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AA Sequence
AERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQ
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Molecular Weight
58.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HARS1, or Human Arginyl-tRNA Synthetase 1, is an essential enzyme involved in the translation of proteins by facilitating the attachment of arginine to its corresponding tRNA, thereby playing a crucial role in protein synthesis. Research on HARS1 has garnered interest due to its implications in various cellular processes, including stress responses and cell proliferation. Studies have revealed that HARS1 is not only pivotal for normal cellular function but also implicated in several diseases, such as cancer and neurodegenerative disorders. The intricate regulatory mechanisms that govern HARS1 expression and activity continue to be a topic of investigation, as researchers seek to understand its dual role in promoting survival under stress conditions while potentially contributing to pathogenesis when dysregulated. Recent advancements in recombinant protein technology have enabled the production of HARS1 in the laboratory, allowing for detailed analysis of its structure-function relationships. Understanding the biochemical properties of HARS1 and its interactions with other cellular components can provide insights into its functional mechanisms and therapeutic potential. Furthermore, exploring the modulation of HARS1 activity may offer novel avenues for developing targeted treatments for diseases linked to its dysfunction. As the field continues to evolve, comprehensive studies on HARS1 and its recombinant forms could illuminate its significance in health and disease, ultimately contributing to advancements in biomedical research and therapeutics.
