Analytical Data
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基因名
PIWIL1
- Application
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别名
HIWI; MIWI; Piwi (Drosophila) like 1; PIWI; Piwi homolog; Piwi like 1 (drosophila); Piwi like 1; Piwi-like protein 1; PIWIL1; PIWL1_HUMAN
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96J94
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表达区间
431-541 aa
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氨基酸序列
NDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVDDRTEAY
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分子量
37.95 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIWIL1, a member of the PIWI (P-element Induced Wimpy Testis) protein family, plays a crucial role in the regulation of germline development and the maintenance of stem cell functions. It is a critical component of the piRNA (PIWI-interacting RNA) pathway, which is essential for safeguarding the genome integrity by silencing transposable elements and preventing their mobilization in germ cells. The study of PIWIL1 is particularly significant in the context of reproductive biology and cancer research, as alterations in its expression and function have been implicated in various forms of cancer and infertility. Researchers have increasingly focused on the recombinant expression of PIWIL1 to explore its functional properties and mechanisms of action. By producing PIWIL1 as a recombinant protein, scientists aim to investigate its interactions with piRNAs and other cellular partners, shedding light on its role in epigenetic regulation and germline stem cell maintenance. Additionally, recombinant PIWIL1 can serve as a valuable tool in developing therapeutic interventions for diseases associated with PIWI family proteins. Understanding the structure and function of PIWIL1 contributes to a broader comprehension of the molecular basis governing gametogenesis and tumorigenesis, ultimately paving the way for innovative diagnostic and treatment strategies.
