Analytical Data
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Gene name
GAPDHS
- Application
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Alternative Names
GAPDHS；GAPD2；GAPDH2；GAPDS；Glyceraldehyde-3-phosphate dehydrogenase. testis-specific
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14556
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Expression Region
69-408aa
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AA Sequence
MVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
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Molecular Weight
39.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic) is a crucial enzyme primarily expressed in the testis, playing a pivotal role in male fertility through its involvement in glycolysis and energy metabolism during sperm development. Its unique nucleotide sequence and distinct regulatory mechanisms set it apart from the ubiquitous GAPDH isoforms found in other tissues. Recent research has highlighted the enzyme's potential beyond metabolism, suggesting roles in apoptosis, cellular signaling, and the regulation of gene expression. Given that disturbances in GAPDHS expression can lead to male infertility, the protein has emerged as a significant focus of study in reproductive biology. Researchers are particularly interested in the structural and functional characterization of GAPDHS to understand its specific contributions to spermatogenesis and to explore its potential as a biomarker for male reproductive health. Additionally, the development of GAPDHS recombinant proteins facilitates the study of its enzymatic properties and interactions with other cellular components, thereby paving the way for novel therapeutic approaches to address fertility issues. Understanding the functional dynamics of GAPDHS not only enriches our knowledge of sperm biology but also opens avenues for targeted interventions in cases of unexplained infertility.
