Analytical Data
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Gene name
PIP5KL1
- Application
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Alternative Names
PIP5KL1; Phosphatidylinositol 4-phosphate 5-kinase-like protein 1; PI(4)P 5-kinase-like protein 1; PtdIns(4)P-5-kinase-like protein 1; EC 2.7.1.68
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5T9C9
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Expression Region
1-394 aa
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AA Sequence
MAAPSPGPRE VLAPSPEAGC RAVTSSRRGL LWRLRDKQSR LGLFEISPGH ELHGMTCMMQ AGLWAATQVS MDHPPTGPPS RDDFSEVLTQ VHEGFELGTL AGPAFAWLRR SLGLAEEDYQ AALGPGGPYL QFLSTSKSKA SFFLSHDQRF FLKTQGRREV QALLAHLPRY VQHLQRHPHS LLARLLGVHS LRVDRGKKTY FIVMQSVFYP AGRISERYDI KGCEVSRWVD PAPEGSPLVL VLKDLNFQGK TINLGPQRSW FLRQMELDTT FLRELNVLDY SLLIAFQRLH EDERGPGSSL IFRTARSVQG AQSPEESRAQ NRRLLPDAPN ALHILDGPEQ RYFLGVVDLA TVYGLRKRLE HLWKTLRYPG RTFSTVSPAR YARRLCQWVE AHTE
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Molecular Weight
44.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIP5KL1 (Phosphatidylinositol-4,5-bisphosphate 5-kinase-like 1) is a member of the phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) signaling pathway, which plays a crucial role in cellular processes such as cell signaling, membrane dynamics, and cytoskeletal organization. The gene encoding PIP5KL1 has gained interest due to its potential involvement in various physiological processes and diseases, including cancer and neurodegeneration. Studies suggest that PIP5KL1 may influence the production of PtdIns(4,5)P2, a key phospholipid that regulates multiple intracellular signaling pathways, including those involved in cell migration, growth, and differentiation. Understanding the structural and functional characteristics of PIP5KL1 through recombinant protein studies can provide insights into its enzymatic activity, regulatory mechanisms, and interactions with other signaling molecules. Moreover, given its potential role in pathological conditions, PIP5KL1 represents a promising target for therapeutic interventions. The production of recombinant PIP5KL1 protein enables researchers to study its biophysical properties, catalyze specific biochemical reactions, and explore its interaction with cellular partners. This approach offers a more refined understanding of its functions and mechanisms, potentially leading to novel strategies for modulating its activity in disease contexts. As the research surrounding PIP5KL1 continues to evolve, elucidating its structure-function relationships will contribute to the broader understanding of phosphoinositide signaling in health and disease.
