Analytical Data
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基因名
cyp51
- Application
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别名
cyp51；CYP51；Lanosterol 14-alpha demethylase
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种属
Mouse
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8K0C4
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表达区间
1-503aa
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氨基酸序列
MVLLGLLQSGGWVLGQAMEQVTGGNLLSTLLIACAFTLSLVYLFRLAVGHMVQLPAGAKSPPHIYSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEEVYGRLTTPVFGKGVAYDVPNAIFLEQKKIIKSGLNIAHFKQYVPIIEKEAKEYFQSWGESGERNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFTHAAWLLPAWLPLPSFRRRDRAHREIKNIFYKAIQKRRLSKEPAEDILQTLLDSTYKDGRPLTDEEISGMLIGLLLAGQHTSSTTSAWMGFFLAKDKPLQEKCYLEQKAVCGEDLPPLTYDQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWAERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCVGENFAYVQIKTIWSTMLRLYEFDLINGYFPTVNYTTMIHTPENPVIRYKRRSK
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分子量
56.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cytochrome P450 51 (CYP51) is a crucial enzyme involved in sterol biosynthesis, playing a significant role in the production of important cellular components such as cholesterol and various sterols in fungi and other organisms. Its study has gained importance due to its association with antifungal resistance and the development of novel antifungal agents, as CYP51 is a key target for many currently used antifungal drugs. In fungi, CYP51 is critical for synthesizing ergosterol, an essential component of fungal cell membranes. The overexpression or mutation of CYP51 can lead to resistance against azole antifungals, complicating treatment strategies for fungal infections. By understanding the structure and function of CYP51 through recombinant protein studies, researchers aim to elucidate its mechanism of action and interactions with inhibitors, which could pave the way for the design of more effective antifungal therapies. Furthermore, the exploration of different CYP51 isoforms across various species can provide insights into evolutionary adaptation and metabolic diversity. Therefore, the recombinant expression and characterization of CYP51 are pivotal in advancing our understanding of fungal biology and addressing the global challenge of antifungal resistance.
