Analytical Data
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Gene name
PHYHD1
- Application
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Alternative Names
MGC16638; OTTHUMP00000022302; OTTHUMP00000022303; OTTHUMP00000022304; OTTHUMP00000022305; OTTHUMP00000199642; PHYD1_HUMAN; Phyhd1; Phytanoyl CoA dioxygenase domain containing 1; Phytanoyl CoA dioxygenase domain containing protein 1; Phytanoyl-CoA dioxygenase domain-containing protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5SRE7
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Expression Region
1-291 aa
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AA Sequence
MACLSPSQLQ KFQQDGFLVL EGFLSAEECV AMQQRIGEIV AEMDVPLHCR TEFSTQEEEQ LRAQGSTDYF LSSGDKIRFF FEKGVFDEKG NFLVPPEKSI NKIGHALHAH DPVFKSITHS FKVQTLARSL GLQMPVVVQS MYIFKQPHFG GEVSPHQDAS FLYTEPLGRV LGVWIAVEDA TLENGCLWFI PGSHTSGVSR RMVRAPVGSA PGTSFLGSEP ARDNSLFVPT PVQRGALVLI HGEVVHKSKQ NLSDRSRQAY TFHLMEASGT TWSPENWLQP TAELPFPQLY T
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Molecular Weight
32.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PHYHD1, a protein encoded by the PHYA gene in plants, plays a crucial role in the phytochrome signaling pathways involved in plant responses to light. This photoreceptor is known to mediate various physiological processes, including seed germination, shade avoidance, and flowering time regulation, by perceiving red and far-red light. The study of PHYHD1 recombinant protein is significant for understanding its structural and functional properties, particularly in light-mediated plant development. Advances in recombinant DNA technology allow for the production of large amounts of pure PHYHD1 protein, facilitating in-depth biochemical analyses and functional assays. Investigating PHYHD1's interactions with other plant proteins and its specific roles in light signaling pathways can provide insights into its regulatory mechanisms. This knowledge is not only vital for fundamental plant biology but also has potential agricultural implications, such as improving crop resilience to varying light conditions and optimizing growth traits. Additionally, the recombinant expression of PHYHD1 in model organisms can aid in understanding its evolutionary conservation and functions across different species, emphasizing the importance of phytochrome in plant adaptation to environmental changes.
