Analytical Data
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基因名
ATP6V1D
- Application
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别名
ATP6V1D; ATP6M; VATD; V-type proton ATPase subunit D
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y5K8
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表达区间
1-247aa
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氨基酸序列
MSGKDRIEIF PSRMAQTIMK ARLKGAQTGR NLLKKKSDAL TLRFRQILKK IIETKMLMGE VMREAAFSLA EAKFTAGDFS TTVIQNVNKA QVKIRAKKDN VAGVTLPVFE HYHEGTDSYE LTGLARGGEQ LAKLKRNYAK AVELLVELAS LQTSFVTLDE AIKITNRRVN AIEHVIIPRI ERTLAYIITE LDEREREEFY RLKKIQEKKK ILKEKSEKDL EQRRAAGEVL EPANLLAEEK DEDLLFE
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分子量
52.91 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP6V1D is a vital subunit of the V-ATPase (vacuolar-type H+-ATPase) complex, which plays a crucial role in various cellular processes by mediating the transport of protons across cellular membranes. This proton pump is essential for maintaining pH homeostasis, regulating ion gradients, and facilitating nutrient uptake in eukaryotic cells. Research has shown that V-ATPase, and specifically the ATP6V1D subunit, is involved in diverse physiological functions including endocytosis, receptor-mediated signaling, and bone resorption, indicating its importance in both normal cellular function and pathological conditions such as cancer and osteoporosis. The overexpression or dysregulation of ATP6V1D has been associated with tumor progression and metastasis, making it a potential target for therapeutic intervention. To better understand its function and implications in disease, recombinant ATP6V1D protein is often studied in vitro and in vivo, enabling researchers to elucidate its molecular mechanisms and develop strategies for modulating its activity. The production and study of this recombinant protein are therefore critical for advancing our knowledge of V-ATPase's role in health and disease.
