Analytical Data
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基因名
Taq
- Application
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别名
Taq；Coagulation factor IX
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19821
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表达区间
293-832aa
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氨基酸序列
ALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERKAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE
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分子量
61.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Taq polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus, discovered in the late 1960s, which gained prominence in molecular biology due to its critical role in the polymerase chain reaction (PCR). PCR, invented by Kary Mullis in 1983, revolutionized genetic research by allowing the amplification of specific DNA sequences, enabling a myriad of applications such as cloning, gene expression analysis, and forensic science. The inherent stability of Taq polymerase at high temperatures is essential for denaturing DNA strands during PCR cycles, thereby facilitating the enzymatic synthesis of new DNA strands. Over the years, extensive research has focused on the modification and optimization of Taq polymerase to enhance its fidelity, processivity, and heat tolerance. Variants of Taq polymerase have been engineered to minimize error rates during DNA synthesis, making them suitable for applications requiring high accuracy. Furthermore, studies have explored the enzyme's mechanisms of action and interactions with DNA, paving the way for the development of next-generation polymerases with improved characteristics. The ongoing research into Taq polymerase and its derivatives continues to advance not only the capabilities of PCR but also the broader field of molecular biology, contributing to innovations in diagnostics, therapeutic development, and genetic engineering.
