Analytical Data
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基因名
ATP6V0A1
- Application
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别名
ATP6N1; ATP6N1A; ATP6V0A1; ATPase H+ transporting lysosomal (vacuolar proton pump)
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q93050
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表达区间
1-831aa
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氨基酸序列
MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFDEMADPDLLEESSSLLEPSEMGRGTPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNIVDAYGIGTYREINPAPYTIITFPFLFAVMFGDFGHGILMTLFAVWMVLRESRILSQKNENEMFSTVFSGRYIILLMGVFSMYTGLIYNDCFSKSLNIFGSSWSVRPMFTYNWTEETLRGNPVLQLNPALPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNHIYFKKPLNIYFGFIPEIIFMTSLFGYLVILIFYKWTAYDAHTSENAPSLLIHFINMFLFSYPESGYSMLYSGQKGIQCFLVVVALLCVPWMLLFKPLVLRRQYLRRKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHSEDADEFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVIHIGLSVKSLAGGLVLFFFFTAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGTGFKFLPFSFEHIREGKFEE
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分子量
122.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP6V0A1, a crucial component of the vacuolar H^+-ATPase (V-ATPase) complex, plays a significant role in acidic intracellular compartments and is pivotal for various cellular processes, including protein degradation, nutrient sensing, and pH regulation. The V-ATPase complex is a multi-subunit enzyme that utilizes ATP hydrolysis to pump protons across membranes, thereby establishing and maintaining acidic environments necessary for proper organelle function. Dysregulation of ATP6V0A1 has been implicated in several pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders, making it a potential therapeutic target. The study of recombinant ATP6V0A1 protein is essential for understanding its structural and functional properties, enabling researchers to elucidate its role in cellular homeostasis and disease etiology. Additionally, producing recombinant ATP6V0A1 can facilitate high-throughput screening of inhibitors or modulators that target V-ATPase activity, contributing to the development of novel treatment strategies. By employing various expression systems and purification techniques, researchers aim to acquire sufficient quantities of functional ATP6V0A1 for biochemical and biophysical characterization, paving the way for advanced studies into its mechanism of action and interaction with other cellular components. Overall, research on recombinant ATP6V0A1 protein is fundamental in advancing our knowledge of V-ATPase's role in health and disease, with implications for both basic research and clinical applications.
