Analytical Data
-
Gene name
ATP5A1
- Application
-
Alternative Names
ATP5A; Atp5a1; ATP5AL2; ATPA_HUMAN; ATPM
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P25705
-
Expression Region
1-553aa
-
AA Sequence
MLSVRVAAAVVRALPRRAGLVSRNALGSSFIAARNFHASNTHLQKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA
-
Molecular Weight
86.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ATP5A1, a crucial component of the mitochondrial ATP synthase complex, plays a fundamental role in cellular energy production by catalyzing the synthesis of adenosine triphosphate (ATP) during oxidative phosphorylation. Its significance extends beyond energy metabolism, as emerging research suggests that ATP5A1 may also be involved in various cellular processes, including apoptosis, cellular signaling, and the regulation of mitochondrial dynamics. Given its central role in bioenergetics and potential implications in metabolic disorders, cancer, and neurodegenerative diseases, the study of recombinant ATP5A1 protein has gained substantial interest. Researchers aim to produce this protein through recombinant techniques to investigate its structure, function, and interactions with other mitochondrial components. Understanding the precise mechanisms by which ATP5A1 contributes to ATP synthesis and its broader biological activities could provide insights into pathological conditions where mitochondrial dysfunction is a hallmark. Furthermore, recombinant ATP5A1 may serve as a valuable tool for drug screening and development, particularly in targeting mitochondrial pathways to enhance cellular energy production or combat diseases linked to mitochondrial impairment. Overall, the exploration of ATP5A1 recombinant protein represents a promising avenue for advancing our knowledge of mitochondrial biology and developing potential therapeutic strategies.
