Analytical Data
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Gene name
USP15
- Application
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Alternative Names
USP15；KIAA0529；Ubiquitin carboxyl-terminal hydrolase 15
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y4E8-4
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Expression Region
1-235aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMAEGGAADLDTQRSDIATLLKTSLRKG DTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQ SLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHC KVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKET RLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTP KKPLEQSC
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Molecular Weight
30 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USP15 (Ubiquitin-specific protease 15) is a deubiquitinating enzyme that plays a critical role in regulating protein stability and cellular signaling pathways by removing ubiquitin molecules from target proteins. Dysregulation of USP15 has been implicated in various diseases, including cancer, where it can influence tumor progression by stabilizing pro-oncogenic factors or degrading tumor suppressors. Recent studies have demonstrated that USP15 impacts multiple signaling pathways, including the NF-κB and TGF-β pathways, making it a potential therapeutic target. The production and characterization of recombinant USP15 protein are essential for understanding its biochemical properties, substrate specificity, and functional mechanisms. By utilizing techniques such as recombinant DNA technology, researchers can generate USP15 in sufficient quantities for enzymatic assays and structural studies. This research is aimed at elucidating the precise role of USP15 in cellular homeostasis and its implications in disease, which could pave the way for the development of new therapeutic strategies targeting USP15-mediated pathways.
