Analytical Data
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基因名
PABPC1
- Application
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别名
PABPC1；PAB1；PABP；PABP1；Polyadenylate-binding Protein 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P11940
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表达区间
1-370aa
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氨基酸序列
MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQR
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分子量
57.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PABPC1 (Poly(A)-binding protein cytoplasmic 1) is a crucial RNA-binding protein that plays a significant role in the regulation of mRNA metabolism, including stability, translation, and degradation. It binds to the poly(A) tail of mRNAs, influencing their translational efficiency and half-life. Research on PABPC1 has surged due to its involvement in various cellular processes and its implications in diseases such as cancer and neurodegenerative disorders. Understanding the functional mechanisms of PABPC1 is vital for elucidating its role in gene expression regulation. Recombinant PABPC1 is often produced to study its structure-function relationships, interaction with other proteins, and its impact on mRNA processing. Techniques such as cloning, expression in suitable systems (like E. coli or insect cells), and purification are employed to obtain functional recombinant protein for biochemical and biophysical analyses. These studies not only advance our understanding of PABPC1’s contributions to cellular physiology but also hold potential for therapeutic interventions targeting mRNA regulation.
