Analytical Data
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基因名
ATE1
- Application
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别名
AI225793; Arginine tRNA Protein transferase 1; Arginine-tRNA--Protein transferase 1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95260
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表达区间
1-518aa
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氨基酸序列
MAFWAGGSPSVVDYFPSEDFYRCGYCKNESGSRSNGMWAHSMTVQDYQDLIDRGWRRSGKYVYKPVMNQTCCPQYTIRCRPLQFQPSKSHKKVLKKMLKFLAKGEVPKGSCEDEPMDSTMDDAVAGDFALINKLDIQCDLKTLSDDIKESLESEGKNSKKEEPQELLQSQDFVGEKLGSGEPSHSVKVHTVPKPGKGADLSKPPCRKAKEIRKERKRLKLMQQNPAGELEGFQAQGHPPSLFPPKAKSNQPKSLEDLIFESLPENASHKLEVRLVPVSFEDPEFKSSFSQSFSLYVKYQVAIHQDPPDECGKTEFTRFLCSSPLEAETPPNGPDCGYGSFHQQYWLDGKIIAVGVIDILPNCVSSVYLYYDPDYSFLSLGVYSALREIAFTRQLHEKTSQLSYYYMGFYIHSCPKMKYKGQYRPSDLLCPETYVWVPIEQCLPSLENSKYCRFNQDPEAVDEDRSTEPDRLQVFHKRAIMPYGVYKKQQKDPSEEAAVLQYASLVGQKCSERMLLFRN
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分子量
83.38 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATE1 (Aminoacyl-tRNA Enterase 1) is an essential enzyme involved in the post-translational modification of proteins. It catalyzes the addition of aminoacyl groups to specific tRNA molecules, facilitating the correct incorporation of amino acids into nascent polypeptide chains during translation. The AT1 protein plays a critical role in cellular stress responses, particularly in the stress granule formation and maintenance, which are crucial for cell survival under adverse conditions. Research has shown that ATE1 is implicated in various cellular processes, including gene expression regulation, protein quality control, and apoptosis. Mutations or dysregulation of ATE1 have been linked to several diseases, including neurodegenerative disorders and cancer. As such, understanding the structure and function of ATE1, as well as its interactions with other cellular components, is vital for uncovering the molecular mechanisms underlying these conditions and for developing potential therapeutic strategies. The study of ATE1-recombinant proteins has emerged as a powerful approach to investigate its enzymatic activity and regulatory mechanisms in detail, enabling researchers to elucidate its roles in health and disease, and offering insights into potential avenues for drug development or intervention in related disorders.
