Analytical Data
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Gene name
UPRT
- Application
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Alternative Names
UPRT；Uracil phosphoribosyltransferase homolog
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96BW1
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Expression Region
1-309aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMATELQC PDSMPCHNQQ VNSASTPSPE QLRPGDLILD HAGGNRASRA KVILLTGYAH SSLPAELDSG ACGGSSLNSE GNSGSGDSSS YDAPAGNSFL EDCELSRQIG AQLKLLPMND QIRELQTIIR DKTASRGDFM FSADRLIRLV VEEGLNQLPY KECMVTTPTG YKYEGVKFEK GNCGVSIMRS GEAMEQGLRD CCRSIRIGKI LIQSDEETQR AKVYYAKFPP DIYRRKVLLM YPILSTGNTV IEAVKVLIEH GVQPSVIILL SLFSTPHGAK SIIQEFPEIT ILTTEVHPVA PTHFGQKYFG TD
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Molecular Weight
36 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of UPRT (uracil phosphoribosyltransferase) recombinant protein has gained significance in recent years due to its potential applications in biotechnology and pharmacology. UPRT is an enzyme involved in the nucleotide salvage pathway, specifically converting uracil and phosphoribosyl pyrophosphate (PRPP) into uridine monophosphate (UMP). This process is crucial for maintaining nucleotide balance in cells, especially in organisms that cannot efficiently synthesize nucleotides de novo. The recombinant expression of UPRT provides a means to produce the enzyme in large quantities for downstream applications, including drug development and enzyme-based therapies. Moreover, UPRT is of particular interest in the context of certain cancer treatments, as it can be exploited in the development of prodrug strategies, where uracil analogs are converted into cytotoxic compounds within tumor cells. The understanding of UPRT's structure and function through recombinant protein studies also sheds light on its role in various metabolic pathways and may lead to innovative solutions for targeting specific diseases. Thus, the research on UPRT recombinant protein serves not only to enhance our understanding of cellular metabolism but also to inspire novel therapeutic approaches, emphasizing its relevance in contemporary biomedical research.
