Analytical Data
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Gene name
ATP5a
- Application
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Alternative Names
ATP5a；ATP5A；ATP5A1；ATP5AL2；ATP synthase subunit alpha. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P25705
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Expression Region
44-553aa
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AA Sequence
QKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAE EMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVG EELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGI KAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLY CIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSG CSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFY LHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQI FLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREV AAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVR GYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEI VTNFLAGFEA
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Molecular Weight
71 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP5a, a crucial subunit of the ATP synthase complex in mitochondria, plays a vital role in cellular energy production through oxidative phosphorylation. Research has increasingly focused on ATP5a due to its implication in various diseases, including neurodegenerative disorders and metabolic syndromes. The ATP5a protein helps facilitate the conversion of ADP and inorganic phosphate to ATP, the primary energy currency of the cell, making it essential for numerous biological processes. In recent years, recombinant ATP5a has been produced to study its structure and function, allowing scientists to investigate its interactions within the ATP synthase complex and its regulatory mechanisms. The recombinant protein serves as a valuable tool for understanding mitochondrial dysfunction and its contribution to pathophysiological conditions. Furthermore, studies on ATP5a have provided insights into potential therapeutic targets for enhancing mitochondrial function and improving energy metabolism in affected cells. As research progresses, the exploration of ATP5a's role in cellular bioenergetics continues to reveal critical information about mitochondrial biology and its broader implications in health and disease. The generation of recombinant ATP5a not only aids in elucidating the underlying mechanisms of mitochondrial disorders but also paves the way for innovative therapeutic strategies aimed at enhancing cellular energy production and restoring normal mitochondrial function.
