Analytical Data
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Gene name
PAF1
- Application
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Alternative Names
RNA polymerase II-associated factor 1 homolog. hPAF1. Pancreatic differentiation protein 2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N7H5
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Expression Region
1-531 aa
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AA Sequence
MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAQGGSDNDSDSGSNGGGQRSRSHSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD
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Molecular Weight
86.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PAF1 (Polymerase Associated Factor 1) is a crucial multi-subunit complex involved in the regulation of gene expression, DNA transcription, and RNA processing. Initially identified as a partner of RNA polymerase II, PAF1 plays significant roles in various biological processes, including transcription elongation, histone modification, and chromatin remodeling. Research into PAF1 and its associated proteins has gained attention due to their implications in cellular differentiation, development, and various diseases, including cancer. The PAF1 complex is known to interact with multiple transcription factors and is implicated in the recruitment of histone modifiers, highlighting its role in epigenetic regulation. Further elucidation of the mechanisms governing PAF1 function is essential, as dysregulation of its activity has been linked to oncogenic processes and poor prognosis in several cancers. Investigating the structure and dynamics of PAF1 and its interactions can provide insights into its multifaceted roles in gene regulation and offer potential therapeutic avenues for tackling diseases associated with transcriptional dysregulation. As researchers continue to explore PAF1's diverse functions, it is becoming increasingly clear that this protein is a key player in the intricate network of molecular pathways that govern gene expression and cellular homeostasis.
