Analytical Data
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Gene name
APOBEC1
- Application
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Alternative Names
BEDP; C->U-editing enzyme APOBEC-1; CDAR1; EC 3.5.4.; HEPR
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P41238
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Expression Region
1-236aa
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AA Sequence
MTSEKGPSTG DPTLRRRIEP WEFDVFYDPR ELRKEACLLY EIKWGMSRKI WRSSGKNTTN HVEVNFIKKF TSERDFHPSM SCSITWFLSW SPCWECSQAI REFLSRHPGV TLVIYVARLF WHMDQQNRQG LRDLVNSGVT IQIMRASEYY HCWRNFVNYP PGDEAHWPQY PPLWMMLYAL ELHCIILSLP PCLKISRRWQ NHLTFFRLHL QNCHYQTIPP HILLATGLIH PSVAWR
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Molecular Weight
28.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
APOBEC1, a member of the APOBEC (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like) family, plays a crucial role in RNA editing, particularly in the conversion of cytidine to uridine in apolipoprotein B mRNA, which regulates lipid metabolism and contributes to various metabolic disorders. Originally identified for its function in editing mRNA, research has expanded to explore APOBEC1's involvement in immune responses, especially in antiviral defense and host recognition of viral RNA. Additionally, studies have revealed that APOBEC1 can influence genomic stability through its deaminase activity, which has implications in cancer biology, as aberrant activity may lead to mutagenesis and tumor development. Understanding the structural and functional characteristics of recombinant APOBEC1 is pivotal for elucidating its mechanisms of action and potential therapeutic targeting. Researchers aim to investigate the protein's enzymatic activity, substrate specificity, and interaction with other cellular factors, paving the way for innovative approaches in treating metabolic disorders and malignancies associated with APOBEC1 dysregulation. This multifaceted research approach highlights the significance of detailed studies on recombinant APOBEC1 to unlock its potential in both basic and translational science.











