Analytical Data
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Gene name
ERCC8
- Application
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Alternative Names
ERCC8；CKN1；CSA；DNA excision repair Protein ERCC-8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13216
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Expression Region
1-396aa
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AA Sequence
MLGFLSARQT GLEDPLRLRR AESTRRVLGL ELNKDRDVER IHGGGINTLD IEPVEGRYML SGGSDGVIVL YDLENSSRQS YYTCKAVCSI GRDHPDVHRY SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQTADVFNF EETVYSHHMS PVSTKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRYDY ILATASADSR VKLWDVRRAS GCLITLDQHN GKKSQAVESA NTAHNGKVNG LCFTSDGLHL LTVGTDNRMR LWNSSNGENT LVNYGKVCNN SKKGLKFTVS CGCSSEFVFV PYGSTIAVYT VYSGEQITML KGHYKTVDCC VFQSNFQELY SGSRDCNILA WVPSLYEPVP DDDETTTKSQ LNPAFEDAWS SSDEEG
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ERCC8, also known as Cockayne syndrome protein B (CSB), plays a crucial role in the DNA repair mechanism, particularly in the sub-pathway of nucleotide excision repair (NER) responsible for repairing transcription-coupled DNA damage. Mutations in the ERCC8 gene are associated with Cockayne syndrome, a rare genetic disorder characterized by growth failure, neurodegeneration, and photosensitivity. Research on ERCC8 reconstitution proteins has gained attention as they provide insights into the molecular mechanisms underlying NER and associated disorders. Understanding the functional dynamics of ERCC8 and its interactions with other repair proteins could unveil the pathways contributing to the pathology of Cockayne syndrome and similar conditions. Additionally, ERCC8's involvement in cellular responses to oxidative stress and its potential roles in age-related diseases have broadened the scope of its study. Overall, the exploration of ERCC8 reconstitution proteins offers promising avenues for therapeutic development and enhances our understanding of DNA repair processes that are pivotal for maintaining genomic stability and preventing various diseases.
