Analytical Data
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基因名
PCA
- Application
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别名
PCA;LDLP;Leupaxin
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O60711
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表达区间
1-386aa
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氨基酸序列
MEELDALLEELERSTLQDSDEYSNPAPLPLDQHSRKETNLDETSEILSIQDNTSPLPAQLVYTTNIQELNVYSEAQEPKESPPPSKTSAAAQLDELMAHLTEMQAKVAVRADAGKKHLPDKQDHKASLDSMLGGLEQELQDLGIATVPKGHCASCQKPIAGKVIHALGQSWHPEHFVCTHCKEEIGSSPFFERSGLAYCPNDYHQLFSPRCAYCAAPILDKVLTAMNQTWHPEHFFCSHCGEVFGAEGFHEKDKKPYCRKDFLAMFSPKCGGCNRPVLENYLSAMDTVWHPECFVCGDCFTSFSTGSFFELDGRPFCELHYHHRRGTLCHGCGQPITGRCISAMGYKFHPEHFVCAFCLTQLSKGIFREQNDKTYCQPCFNKLFPL
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分子量
43.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PCA (Protein Complementation Assay) recombinant proteins have emerged as a powerful tool in molecular biology, primarily for studying protein-protein interactions, cellular localization, and functional analysis of proteins in living cells. The technique is based on the principle that two non-fluorescent protein fragments can complement each other to form a functional protein when brought into close proximity, thus allowing researchers to visualize interactions in real-time. This method offers significant advantages over traditional co-immunoprecipitation or pull-down assays, as it enables the detection of interactions in their native cellular environment without the need for overexpression of the proteins involved. Furthermore, PCA can be adapted for use in high-throughput screening applications, facilitating the identification of novel protein interactions on a large scale. The continual refinement of PCA techniques, coupled with advances in recombinant protein technology, has revolutionized our understanding of cellular processes and the complex networks of protein interactions that underpin them. As research delves deeper into the roles of specific proteins in various biological pathways, PCA recombinant proteins are becoming invaluable in elucidating the mechanisms of diseases, developing targeted therapies, and advancing synthetic biology. This burgeoning field promises to uncover new insights into the dynamic nature of cellular functions and the intricate choreography of molecular interactions that sustain life.












