Analytical Data
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基因名
NPHP5
- Application
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别名
NPHP5；KIAA0036；NPHP5；IQ calmodulin-binding motif-containing Protein 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15051
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表达区间
1-598aa
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氨基酸序列
MKPTGTDPRI LSIAAEVAKS PEQNVPVILL KLKEIINITP LGSSELKKIK QDIYCYDLIQ YCLLVLSQDY SRIQGGWTTI SQLTQILSHC CVGLEPGEDA EEFYNELLPS AAENFLVLGR QLQTCFINAA KAEEKDELLH FFQIVTDSLF WLLGGHVELI QNVLQSDHFL HLLQADNVQI GSAVMMMLQN ILQINSGDLL RIGRKALYSI LDEVIFKLFS TPSPVIRSTA TKLLLLMAES HQEILILLRQ STCYKGLRRL LSKQETGTEF SQELRQLVGL LSPMVYQEVE EQKLHQAACL IQAYWKGFQT RKRLKKLPSA VIALQRSFRS KRSKMLLEIN RQKEEEDLKL QLQLQRQRAM RLSRELQLSM LEIVHPGQVE KHYREMEEKS ALIIQKHWRG YRERKNFHQQ RQSLIEYKAA VTLQRAALKF LAKCRKKKKL FAPWRGLQEL TDARRVELKK RVDDYVRRHL GSPMSDVVSR ELHAQAQERL QHYFMGRALE ERAQQHREAL IAQISTNVEQ LMKAPSLKEA EGKEPELFLS RSRPVAAKAK QAHLTTLKHI QAPWWKKLGE ESGDEIDVPK DELSIELENL FIGGTKPP
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分子量
68.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of recombinant NPHP5 protein is rooted in the investigation of nephronophthisis, a genetic disorder characterized by progressive kidney dysfunction and often leading to end-stage renal disease. NPHP5 is one of several genes associated with this condition, and it plays a crucial role in renal ciliopathies, affecting the structure and function of primary cilia in kidney cells. Given its involvement in cellular signaling and regulation of nephron development, understanding the molecular mechanisms of NPHP5 is vital for uncovering potential therapeutic targets. The production of recombinant NPHP5 protein has become a focal point for researchers aiming to elucidate its function, interactions with other proteins, and role in the pathogenesis of nephronophthisis. By generating a pure form of NPHP5, scientists can conduct biochemical and biophysical studies to assess its functional properties, explore potential genetic variants, and develop assays to test the impact of these variants in cellular models. Moreover, recombinant protein studies can facilitate the design of gene therapies or pharmacological interventions aimed at mitigating the effects of NPHP5 mutations, thereby providing insights into targeted treatment strategies for individuals affected by this debilitating condition. Through ongoing research into recombinant NPHP5 protein, the scientific community hopes to advance the understanding of kidney diseases and improve patient outcomes.
