Analytical Data
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Gene name
AKT1S1
- Application
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Alternative Names
AKT1 substrate 1; AKT1S 1; AKT1S1; AKTS1_HUMAN; Lobe; MGC2865; PRAS 40
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96B36
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Expression Region
1-256aa
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AA Sequence
MASGRPEELW EAVVGAAERF RARTGTELVL LTAAPPPPPR PGPCAYAAHG RGALAEAARR CLHDIALAHR AATAARPPAP PPAPQPPSPT PSPPRPTLAR EDNEEDEDEP TETETSGEQL GISDNGGLFV MDEDATLQDL PPFCESDPES TDDGSLSEET PAGPPTCSVP PASALPTQQY AKSLPVSVPV WGFKEKRTEA RSSDEENGPP SSPDLDRIAA SMRALVLREA EDTQVFGDLP RPRLNTSDFQ KLKRKY
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Molecular Weight
27.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AKT1S1, also known as AKT1 substrate 1, plays a crucial role in cellular signaling pathways, particularly those related to the AKT pathway, which is integral for regulating cell metabolism, growth, and survival. This protein serves as a substrate for AKT1, linking the PI3K/Akt signaling cascade to various cellular processes. Research on AKT1S1 has gained momentum due to its implications in several diseases, including cancer, where dysregulation of the AKT pathway is frequently observed. Understanding the functional properties of AKT1S1 and its interactions with other proteins can provide insight into the molecular mechanisms underlying tumorigenesis and contribute to the development of targeted therapies. Moreover, the production of recombinant AKT1S1 protein allows for detailed biochemical studies, including its enzymatic activity, structural analysis, and the identification of its binding partners. This research not only enhances our knowledge of AKT1S1's role in cellular signaling but also opens new avenues for therapeutic interventions aimed at modulating the AKT pathway in various pathological contexts.
