Cat: PA2000-5420

Recombinant Human AKR1CL2 Protein,His

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Analytical Data

  • Gene name

    AKR1CL2

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    AKR1E2; AKR1CL2; AKRDC11.5-anhydro-D-fructose reductase; AF reductase; EC 1.1.1.263

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q96JD6

  • Expression Region

    1-320aa

  • AA Sequence

    MGDIPAVGLS SWKASPGKVT EAVKEAIDAG YRHFDCAYFY HNEREVGAGI RCKIKEGAVR REDLFIATKL WCTCHKKSLV ETACRKSLKA LKLNYLDLYL IHWPMGFKPP HPEWIMSCSE LSFCLSHPRV QDLPLDESNM VIPSDTDFLD TWEAMEDLVI TGLVKNIGVS NFNHEQLERL LNKPGLRFKP LTNQIECHPY LTQKNLISFC QSRDVSVTAY RPLGGSCEGV DLIDNPVIKR IAKEHGKSPA QILIRFQIQR NVIVIPGSIT PSHIKENIQV FDFELTQHDM DNILSLNRNL RLAMFPITKN HKDYPFHIEY

  • Molecular Weight

    36.5 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

AKR1CL2, a member of the aldo-keto reductase (AKR) superfamily, is an enzyme involved in the metabolism of various endogenous and exogenous compounds, including steroid hormones, catecholamines, and drugs. This enzyme plays a crucial role in regulating physiological processes through the reduction of carbonyl groups in these compounds, which can affect their biological activity and detoxification. Given its potential implications in diseases such as cancer, diabetes, and neurological disorders, researchers have increasingly focused on exploring the functional characteristics of AKR1CL2, its substrate specificity, and its expression patterns in different tissues. The importance of AKR enzymes in pharmacology and toxicology further underscores the need to understand AKR1CL2's role in metabolic pathways. Recent studies utilizing recombinant protein expression technologies have enabled the detailed investigation of AKR1CL2's structure-function relationships, providing insights into its catalytic mechanisms and potential as a therapeutic target. Overall, the study of AKR1CL2 not only enhances our comprehension of metabolic regulation but also opens avenues for novel drug development and disease treatment strategies.

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