Analytical Data
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Gene name
STX4
- Application
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Alternative Names
STX4；STX4A；Syntaxin-4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12846
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Expression Region
1-275aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMRDRT HELRQGDDSS DEEDKERVAL VVHPGTARLG SPDEEFFHKV RTIRQTIVKL GNKVQELEKQ QVTILATPLP EESMKQELQN LRDEIKQLGR EIRLQLKAIE PQKEEADENY NSVNTRMRKT QHGVLSQQFV ELINKCNSMQ SEYREKNVER IRRQLKITNA GMVSDEELEQ MLDSGQSEVF VSNILKDTQV TRQALNEISA RHSEIQQLER SIRELHDIFT FLATEVEMQG EMINRIEKNI LSSADYVERG QEHVKTALEN QKKARKKKVL
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Molecular Weight
35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
STX4, or Syntaxin 4, is a member of the SNARE (Soluble NSF Attachment Protein Receptor) protein family, which plays a crucial role in the membrane fusion process essential for exocytosis and intracellular transport. This protein predominantly localizes to the plasma membrane of various cell types, where it facilitates the fusion of secretory vesicles with the membrane, enabling the release of neurotransmitters, hormones, and other signaling molecules. Research into STX4 has gained momentum due to its implications in various physiological processes and diseases. Mutations or dysregulation of STX4 have been linked to conditions such as diabetes, where impaired insulin secretion from pancreatic beta-cells can occur, and neurological disorders, affecting neurotransmitter release. Additionally, STX4 is involved in the immune response, influencing the secretion of cytokines and other immune mediators. Understanding the structure and function of STX4 at the molecular level is critical for elucidating its role in these processes, providing potential targets for therapeutic intervention. Various studies have employed recombinant protein techniques to express and purify STX4, allowing for in-depth functional assays and structural analyses. Investigating the interaction of STX4 with other SNARE proteins and regulatory factors will enhance our understanding of its role in cellular mechanisms and its potential involvement in diseases, paving the way for the development of targeted therapies aimed at modulating its function. Overall, the ongoing research on STX4 holds promise for advancing our understanding of exocytosis and its broader implications in health and disease.
