Analytical Data
-
基因名
AFF4
- Application
-
别名
AFF4; AF5Q31; MCEF; HSPC092; AF4/FMR2 family member 4
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9UHB7
-
表达区间
1-365aa
-
氨基酸序列
MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQRLK
-
分子量
66.6 KDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AFF4 (AF4/FMR2 family member 4) is a critical protein that plays a significant role in transcriptional regulation and RNA processing, particularly in hematopoietic cells. Its involvement in various cellular processes has made it a focal point of research, especially considering its implications in diseases such as leukemia and other malignancies. AFF4 is known to interact with the super elongation complex (SEC), which facilitates the elongation of RNA polymerase II during transcription. Aberrant expression or mutations of AFF4 can lead to disrupted transcriptional control and contribute to tumorigenesis. Recent studies have highlighted its overexpression in certain types of cancer, which has prompted investigations into its potential as a therapeutic target. Understanding the structure, function, and downstream effects of AFF4 is crucial for elucidating its role in both normal cellular physiology and disease states. The reconstitution of AFF4 and its associated complexes in vitro offers the opportunity to explore its mechanisms of action and interactions with other molecular players in transcription regulation. Given its significance in both developmental biology and oncology, AFF4 has emerged as a promising candidate for further research aimed at developing novel therapeutic strategies against various cancers.
