Analytical Data
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Gene name
AFF4
- Application
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Alternative Names
AFF4; AF5Q31; MCEF; HSPC092; AF4/FMR2 family member 4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UHB7
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Expression Region
1-365aa
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AA Sequence
MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQRLK
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Molecular Weight
66.6 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AFF4 (AF4/FMR2 family member 4) is a critical protein that plays a significant role in transcriptional regulation and RNA processing, particularly in hematopoietic cells. Its involvement in various cellular processes has made it a focal point of research, especially considering its implications in diseases such as leukemia and other malignancies. AFF4 is known to interact with the super elongation complex (SEC), which facilitates the elongation of RNA polymerase II during transcription. Aberrant expression or mutations of AFF4 can lead to disrupted transcriptional control and contribute to tumorigenesis. Recent studies have highlighted its overexpression in certain types of cancer, which has prompted investigations into its potential as a therapeutic target. Understanding the structure, function, and downstream effects of AFF4 is crucial for elucidating its role in both normal cellular physiology and disease states. The reconstitution of AFF4 and its associated complexes in vitro offers the opportunity to explore its mechanisms of action and interactions with other molecular players in transcription regulation. Given its significance in both developmental biology and oncology, AFF4 has emerged as a promising candidate for further research aimed at developing novel therapeutic strategies against various cancers.
