Analytical Data
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基因名
ADSSL1
- Application
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别名
ADSS1; ADSSL1; Adenylosuccinate synthetase isozyme 1; AMPSase 1; AdSS 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N142
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表达区间
1-457aa
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氨基酸序列
MSGTRASNDR PPGAGGVKRG RLQQEAAATG SRVTVVLGAQ WGDEGKGKVV DLLATDADII SRCQGGNNAG HTVVVDGKEY DFHLLPSGII NTKAVSFIGN GVVIHLPGLF EEAEKNEKKG LKDWEKRLII SDRAHLVFDF HQAVDGLQEV QRQAQEGKNI GTTKKGIGPT YSSKAARTGL RICDLLSDFD EFSSRFKNLA HQHQSMFPTL EIDIEGQLKR LKGFAERIRP MVRDGVYFMY EALHGPPKKI LVEGANAALL DIDFGTYPFV TSSNCTVGGV CTGLGIPPQN IGDVYGVVKA YTTRVGIGAF PTEQINEIGG LLQTRGHEWG VTTGRKRRCG WLDLMILRYA HMVNGFTALA LTKLDILDVL GEVKVGVSYK LNGKRIPYFP ANQEMLQKVE VEYETLPGWK ADTTGARRWE DLPPQAQNYI RFVENHVGVA VKWVGVGKSR ESMIQLF
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分子量
50.2 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ADSSL1 (adenylosuccinate lyase 1) is an enzyme involved in the purine nucleotide synthesis pathway, specifically playing a critical role in the conversion of adenylosuccinate to AMP and fumarate. Mutations in the ADSSL1 gene have been linked to adenylyl-succinate lyase deficiency, a rare genetic disorder characterized by neurological manifestations, such as developmental delays and seizures, primarily affecting the central nervous system. Given the enzyme’s importance in nucleotide metabolism, research into ADSSL1 recombinant protein focuses on understanding its structural and functional properties, which can provide insights into disease mechanisms associated with ADSSL1 deficiency. Additionally, studying the recombinant form of ADSSL1 allows for the exploration of potential therapeutic approaches, including enzyme replacement therapies or small molecule drugs that could enhance or restore its activity. Moreover, understanding the enzymatic kinetics and interactions of ADSSL1 may reveal broader implications in metabolic disorders. The advancement of techniques in protein expression and purification further facilitates these studies, making it possible to generate sufficient quantities of the enzyme for in vitro analysis and potential applications in targeted treatments. As such, the research on ADSSL1 recombinant protein not only contributes to the fundamental understanding of purine metabolism but also holds promise for developing new strategies to address metabolic disorders linked to this enzyme.
