Analytical Data
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Gene name
SPAM1
- Application
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Alternative Names
SPAM1；HYAL3；PH20；Hyaluronidase PH-20
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P38567
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Expression Region
1-509aa
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AA Sequence
MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFL WAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYP YIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEW RPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFL VETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLS WLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV FAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKS CLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHL NPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVK DTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILF LIISSVASL
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Molecular Weight
84 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPAM1, also known as sperm protein associated with the nucleus 1, is a protein that has garnered significant attention in reproductive biology due to its role in sperm maturation and fertilization. Research indicates that SPAM1 is primarily expressed in the testes and is involved in the binding of sperm to the zona pellucida, the outer glycoprotein layer of the oocyte, facilitating the fertilization process. Its expression pattern and functional significance suggest that SPAM1 may play a crucial role in male fertility, with potential implications for reproductive health. Studies have also explored its involvement in various reproductive pathologies, making it a potential biomarker for male infertility. The focus on SPAM1’s recombinant protein form aims to elucidate its functional mechanisms and interactions at the molecular level, which could lead to novel therapeutic strategies for treating infertility. Furthermore, the characterization of SPAM1 as a recombinant protein offers opportunities for developing specific antibody-based assays that can enhance our understanding of sperm-egg interaction dynamics. Overall, the exploration of SPAM1’s role in reproduction not only furthers our comprehension of fundamental biological processes but also opens new avenues for addressing fertility challenges in clinical settings.
