Analytical Data
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Gene name
ADAT3
- Application
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Alternative Names
ADAT3; ADAT3_HUMAN; adenosine deaminase; tRNA specific 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96EY9
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Expression Region
1-351aa
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AA Sequence
MEPAPGLVEQ PKCLEAGSPE PEPAPWQALP VLSEKQSGDV ELVLAYAAPV LDKRQTSRLL KEVSALHPLP AQPHLKRVRP SRDAGSPHAL EMLLCLAGPA SGPRSLAELL PRPAVDPRGL GQPFLVPVPA RPPLTRGQFE EARAHWPTSF HEDKQVTSAL AGRLFSTQER AAMQSHMERA VWAARRAAAR GLRAVGAVVV DPASDRVLAT GHDCSCADNP LLHAVMVCVD LVARGQGRGT YDFRPFPACS FAPAAAPQAV RAGAVRKLDA DEDGLPYLCT GYDLYVTREP CAMCAMALVH ARILRVFYGA PSPDGALGTR FRIHARPDLN HRFQVFRGVL EEQCRWLDPD T
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Molecular Weight
38.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ADAT3 (Adenosine Deaminase Acting on tRNA 3) is a crucial enzyme involved in the post-transcriptional modification of tRNA, specifically catalyzing the deamination of adenosine to inosine in tRNA molecules. This modification is vital for maintaining the proper folding and function of tRNA, which in turn is essential for accurate protein synthesis. Research into ADAT3 has gained momentum due to its implications in various biological processes and diseases. Alterations in tRNA modifications, including those mediated by ADAT3, have been associated with conditions such as cancer, neurodegenerative disorders, and metabolic syndromes. Understanding the structure and function of ADAT3 is therefore critical for elucidating the broader impact of tRNA modifications on cellular physiology. Furthermore, the exploration of ADAT3 as a potential therapeutic target has opened avenues for innovative treatments in diseases where the modulation of tRNA activity could restore normal protein synthesis. Advances in recombinant protein technology have allowed for the production and characterization of ADAT3, enabling researchers to investigate its enzymatic properties, regulatory mechanisms, and potential interactions with other cellular components. As the field progresses, ADAT3 represents a promising candidate for further study in the quest to comprehend the intricate relationship between tRNA modifications and human health.
