Cat: PA2000-1257

Recombinant Human FOXD3 Protein,His

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Analytical Data

  • Gene name

    FOXD3

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    FOXD3;HFH2;Forkhead box Protein D3

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9UJU5

  • Expression Region

    2-478aa

  • AA Sequence

    29 amino acid Tag-GEFTLSGGGSASDMSGQTVLTAEDVDIDVVGEGDDGLEEKDSDAGC DSPAGPPEL RLDEADEVPPAAPHHGQPQPPHQQPLTLPKEAAGAGAGP GGDVGAPEADGCKGGVGGEEGGASG GGPGAGSGSAGGLAPSKPKNSLV KPPYSYIALITMAILQSPQKKLTLSGICEFISNRFPYYREK FPAWQNS IRHNLSLNDCFVKIPREPGNPGKGNYWTLDPQSEDMFDNGSFLRRRKRFK RHQQEHL REQTALMMQSFGAYSLAAAAGAAGPYGRPYGLHPAAAAGAY SHPAAAAAAAAAAALQYPYALPP VAPVLPPAVPLLPSGELGRKAAAFG SQLGPGLQLQLNSLGAAAAAAGTAGAAGTTASLIKSEPS ARPSFSIEN IIGGGPAAPGGSAVGAGVAGGTGGSGGGSTAQSFLRPPGTVQSAALMATH QPLSL SRTTATIAPILSVPLSGQFLQPAASAAAAAAAAAQAKWPAQLE ESGGGGSPGRRRRRRRRRRR

  • Molecular Weight

    48 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

FOXD3 (Forkhead box D3) is a member of the Forkhead transcription factor family, which plays a pivotal role in various biological processes, including embryogenesis, cell differentiation, and maintenance of pluripotency in stem cells. Its expression is notably linked to the regulation of key developmental pathways and has been implicated in the processes of neural crest formation and tumorigenesis. In recent years, research has intensified on FOXD3 due to its potential role as a biomarker for certain cancers and its involvement in maintaining stem cell characteristics, particularly in pluripotent stem cells. The production and study of FOXD3 recombinant protein have become essential for elucidating its molecular mechanisms and interactions. Recombinant FOXD3 can be utilized in various experimental settings, including in vitro assays to assess its function in transcriptional regulation, protein-protein interactions, and to investigate its role in inhibiting differentiation of pluripotent cells. Furthermore, understanding the structural and functional properties of FOXD3 through recombinant protein studies can provide critical insights into its involvement in developmental biology and pathology, paving the way for novel therapeutic strategies in regenerative medicine and cancer treatment. Overall, the study of FOXD3 recombinant protein is a crucial step towards deciphering its complex roles in cellular and developmental contexts, ultimately contributing to advancements in understanding stem cell biology and cancer research.

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