Analytical Data
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Gene name
ODF4
- Application
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Alternative Names
ODF4; OPPO1; Outer dense fiber protein 4; Outer dense fiber of sperm tails protein 4; Testis-specific protein oppo 1; hOPPO1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q2M2E3
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Expression Region
1-257 aa
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AA Sequence
MDAEYSGNEFPRSEGERDQHQRPGKERKSGEAGWGTGELGQDGRLLSSTLSLSSNRSLGQRQNSPLPFQWRITHSFRWMAQVLASELSLVAFILLLVVAFSKKWLDLSRSLFYQRWPVDVSNRIHTSAHVMSMGLLHFYKSRSCSDLENGKVTFIFSTLMLFPINIWIFELERNVSIPIGWSYFIGWLVLILYFTCAILCYFNHKSFWSLILSHPSGAVSCSSSFGSVEESPRAQTITDTPITQEGVLDPEQKDTHV
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Molecular Weight
55.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ODF4, or Outer Dense Fiber 4, is a protein primarily expressed in the testis, where it plays a crucial role in the structural integrity and functionality of spermatozoa. Research on ODF4 has gained significant attention due to its involvement in sperm motility and fertility. Observations suggest that ODF4 is part of the outer dense fibers of the sperm tail, contributing to the overall architecture necessary for proper flagellar function. Mutations or dysregulations in the ODF4 gene can lead to impaired sperm formation and various forms of male infertility. Consequently, understanding the molecular mechanisms governing ODF4 expression and function is essential not only for elucidating the basic biology of spermatogenesis but also for potential clinical implications in diagnosing and treating male infertility. Furthermore, studies have indicated that ODF4 may interact with other proteins involved in cytoskeletal organization and cellular signaling pathways, suggesting broader functions beyond reproduction. As research progresses, the characterization of ODF4 and its interactions at the molecular level could offer new insights into reproductive health and potential therapeutic targets for fertility-related disorders. These insights hold promise for improving assisted reproductive technologies and addressing challenges faced by couples experiencing infertility. In summary, ODF4 represents a pivotal molecule in the field of reproductive biology, highlighting the intricate relationship between protein functionality and fertility.
