Analytical Data
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Gene name
NSBP1
- Application
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Alternative Names
High mobility group nucleosome binding domain 5; High mobility group nucleosome-binding domain-containing protein 5; Hmgn5; HMGN5_HUMAN; NBP 45; NSBP 1; NSBP1; Nucleosomal binding protein 1; Nucleosome binding protein 1; Nucleosome-binding protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P82970
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Expression Region
1-282 aa
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AA Sequence
MPKRKAAGQG DMRQEPKRRS ARLSAMLVPV TPEVKPKRTS SSRKMKTKSD MMEENIDTSA QAVAETKQEA VVEEDYNENA KNGEAKITEA PASEKEIVEV KEENIEDATE KGGEKKEAVA AEVKNEEEDQ KEDEEDQNEE KGEAGKEDKD EKGEEDGKED KNGNEKGEDA KEKEDGKKGE DGKGNGEDGK EKGEDEKEEE DRKETGDGKE NEDGKEKGDK KEGKDVKVKE DEKEREDGKE DEGGNEEEAG KEKEDLKEEE EGKEEDEIKE DDGKKEEPQS IV
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Molecular Weight
31.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NSBP1, or Nucleolar and Splicing Factor 1, is a protein of significant interest in molecular biology due to its critical roles in RNA processing and regulation. This protein is predominantly localized in the nucleolus and contributes to the biogenesis of ribosomal RNA and the assembly of ribonucleoprotein complexes. Recent studies have indicated that NSBP1 may be involved in key cellular processes, including cell proliferation and response to stress, which positions it as a potential player in cancer biology. The recombinant expression of NSBP1 allows researchers to investigate its functional implications and interactions with other cellular components more thoroughly. By producing NSBP1 in a controlled environment, scientists can elucidate its role in RNA metabolism and its potential effects on cellular homeostasis. Furthermore, understanding the mechanistic pathways involving NSBP1 could provide insights into various diseases, particularly those related to dysregulated RNA processing. Given its multifaceted roles, the recombinant protein study of NSBP1 is vital for elucidating its functions and developing therapeutic approaches that target its pathways. Overall, research on NSBP1 recombinant protein not only enhances our understanding of fundamental cellular mechanisms but also opens new avenues for tackling diseases associated with its dysfunction.
